Chromosome numbers and karyotypes of four Thai banana varieties from Ratchaburi Province, Thailand
Keywords:
Banana, chromosome, karyotype, idiogramAbstract
This research aims to study the chromosome numbers and karyotypes of four banana varieties from Ratchaburi Province using the root tip squashing technique. The varieties are: Kluai Leb Mu Nang (Musa sapientum Linn.), Kluai Tani (Musa balbisiana Colla.), Kluai Namwa Tanao Sri (Musa × paradisiaca L.), and Kluai Hom Khiew (Musa sp., AAA group "Cavendish"). Chromosomes were obtained from root tips and fixed roots; meristem cells were stained with acetocarmine dye and photographed under a light microscope during the metaphase stage. Data analysis and idiogram display were conducted using Excel and Adobe Photoshop CS5. The results revealed that the chromosome number was 2n=22 for both Kluai Leb Mu Nang and Kluai Tani. For Kluai Namwa Tanao Sri and Kluai Hom Khiew, the chromosome number was 3n=33. Chromosome sizes ranged from 1.30 to 3.40 μm. The karyotypes consisted of metacentric and submetacentric chromosomes, except for Kluai Tani and Kluai Namwa Tanao Sri, which exhibited subtelocentric chromosomes.
References
Chapman, G. P. (1970). Pattern of change in tropical plants. University of London Press.
Cheesman, E. E., & Larter, L. N. H. (1935). Genetical and cytological studies of Musa. III. Chromosome numbers in the Musaceae. Journal of Genetics, 30(1), 31–52. https://doi.org/10.1007/BF02982204
FAOSTAT. (2024). Agriculture Organization of the United Nations, FAO. Available online: http://www.fao.org/home/en/ (accessed on 26 August 2024).
Ferreira, C. F., Silva, S. O., Sobrinho, N. P. D., Cristina, S., Damascena, S., Alves, F. S. D. A. A. O., & da Paz, O. P. (2004). Molecular characterization of banana (AA) diploids with contrasting levels of black and yellow Sigatoka resistance. American Journal of Applied Sciences, 1(4), 276–278. https://doi.org/10.3844/ajassp.2004.276.278
Häkkinen, M. (2013). Reappraisal of sectional taxonomy in Musa (Musaceae). Taxon, 62, 809–813. https://doi.org/10.12705/624.3
Heslop-Harrison, J. S., & Schwarzacher, T. (2007). Domestication, genomics and the future for banana. Annals of Botany, 100(5), 1073–1084. https://doi.org/10.1093/aob/mcm191
Hribova, E., Dolezelova, M., & Dolezel, J. (2008). Localization of BAC clones on mitotic chromosome of Musa acuminata using fluorescence in situ hybridization. Biologia Plantarum, 52(3), 445–452. https://doi.org/10.1007/s10535-008-0089-1
Hutchison, D. J. (1966). Notes on bananas II—Chromosome counts of varieties. Tropical Agriculture, 43(2), 131–132. https://journals.sta.uwi.edu/ojs/index.php/ta/article/view/4712
Janssens, S. B., Vandelook, F., de Langhe, E., Verstraete, B., Smets, E., van den Houwe, I., & Swennen, R. (2016). Evolutionary dynamics and biogeography of Musaceae reveal a correlation between the diversification of the banana family and the geological and climatic history of Southeast Asia. New Phytologist, 210(4), 1453–1465. https://doi.org/10.1111/nph.13856
Levan, A., Fredga, K., & Sandberg, P. (1964). Nomenclature for centromeric position on chromosomes. Hereditas, 52, 201–220. https://doi.org/10.1111/j.1601-5223.1964.tb01953.x
Oselebe, H. O., Tenkouano, A., & Pillay, M. (2006). Ploidy variation of Musa hybrids from crosses. African Journal of Biotechnology, 5(11), 1048–1053.
Phengchang, P., Silayoi, B., Babpraserth, C., & Wongpiyasatid, A. (2009). Karyotype of some bananas in AA and AAA groups in Thailand. Thai Agricultural Research Journal, 27(3), 244–255. https://doi.org/10.14456/thaidoaagres.2009.17
Pitaktharm, T., Phiphitphibunsuk, W., Suwanphakdee, C., & Puangpairote, T. (2024). Chromosome number variation in the genus Acmella (Asteraceae) from Thailand. Cytologia, 89(1), 7–19. https://doi.org/10.1508/cytologia.89.7
Pursglove, J. W. (1978). Musacae: Tropical crop monocotyledon I and II. Longman.
Shu, B. (2000). Musa Linnaeus, Sp. Pl. 2: 1043. 1753. In Flora of China (Vol. 24, pp. 315–318). Science Press & Missouri Botanical Garden Press.
Silayoi, B., & Babpraserth, C. (1983). Banana genetic resource exploration in Thailand. Report submitted to IBPGR, Kasetsart University, Bangkok.
Silayoi, B., & Sompen, P. (1991). Chromosome numbers and karyotypes of some Thai bananas. Agriculture and Natural Resources, 25(4), 400–407. https://li01.tci-thaijo.org/index.php/anres/article/view/241991
Simmonds, N. W., & Shepherd, K. (1955). The taxonomy and origins of the cultivated bananas. Journal of the Linnean Society of London, Botany, 55(359), 302–312. https://doi.org/10.1111/j.1095-8339.1955.tb00015.x
Wu, Z. Y., & Raven, P. H. (Eds.). (2000). Flora of China. Vol. 24 (Musa Linnaeus, Sp. Pl. 2: 1043. 1753. pp. 315–318). Science Press, Beijing, and Missouri Botanical Garden Press, St. Louis.
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2025 Food Agricultural Sciences and Technology
This work is licensed under a Creative Commons Attribution-NoDerivatives 4.0 International License.
