Integrating CRISPR-driven pheromones and RNAi production – Possible “savior” for the management of Nilaparvata lugens in rice paddy felds
Keywords:
Nilaparvata lugens, pheromonetargeted management, RNA interference (RNAi) biopesticides, CRISPR technology, integrated pest management (IPM)Abstract
The brown planthopper (Nilaparvata lugens) is a serious pest affecting rice production worldwide, causing significant economic losses and posing challenges to food security. Traditional and conventional approaches have relied heavily on synthetic insecticides, which have led to increased pest resistance and environmental concerns, highlighting the urgent need for sustainable alternatives. This review discusses innovative strategies that integrate pheromone-targeted approaches and RNA interference (RNAi)-induced biopesticides, enhanced by CRISPR technology. Pheromones, particularly sex pheromones, can disrupt mating behaviours, offering a non-toxic alternative to sustainably reduce pest populations. RNAi biopesticides provide a precision-based solution by silencing essential genes related to pest survival and reproduction, thereby minimizing off-target effects and environmental impacts. Additionally, CRISPR technology enhances these strategies by enabling the synthesis of pheromones independent of insect hosts and facilitating the delivery of RNAi constructs, with potential applications in developing pest-resistant rice varieties. A thorough understanding of the biological and ecological aspects of N. lugens is crucial for evaluating current research on pheromone and RNAi applications within integrated pest management (IPM) frameworks. The challenges and opportunities presented by these innovative approaches necessitate interdisciplinary research to optimize their effectiveness while addressing regulatory and public acceptance concerns. These insights can significantly advance agricultural practices, mitigate rising pest pressures, and enhance both food security and environmental sustainability.
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