Effects of heat treatment on the nutrient composition, phytochemical properties and indices of fat quality of fresh and dried leaves and seeds of Piper guineense (Uziza)

Authors

  • Chukwunwike Uchenna Enyi Department of Food Science and Technology, University of Agriculture and Environmental Science, Umuagwo, P.M.B 1038, Imo State, Nigeria
  • Philippa C. Ojimelukwe Department of Food Science and Technology, Michael Okpara University of Agriculture, Umudike, P.M.B 7267, Umuahia, Abia State, Nigeria
  • Chinasa Okorie-Humphrey Department of Food Science and Technology, Abia State Polytechnic, Aba, Nigeria
  • Onyekachukwu Obumneme Enyi Department of Biotechnology, Ebonyi State University, Abakaliki, P.M.B 501, Ebonyi State, Nigeria
  • Clara Obiageri Ogbedeagu Department of Food Science and Technology, University of Agriculture and Environmental Science, Umuagwo, P.M.B 1038, Imo State, Nigeria
  • Chinedu Norbert Eze Department of Biology, University of Agriculture and Environmental Science, Umuagwo, P.M.B 1038, Imo State, Nigeria

Keywords:

Piper guineense seeds, Piper guineense leaves, carotenoids, nutrients

Abstract

This study evaluated the effect of drying on the nutrient composition, phytochemical constituents, and indices of fat quality of fresh and dried leaves and seeds of Piper guineense (Uziza). The fresh and dried leaves and seeds of P. guineense were analyzed for phytochemical, vitamin, mineral, carotenoid, and quality indices. The results showed that fresh P. guineense leaves had the highest total phenol (1047.33 mg/100g), flavonoid (0.66 mg/100g), zinc (0.42 mg/100g), copper (0.10 mg/100g), vitamin C (2.49 mg/100g) and carotenoid contents while the dried P. guineense seeds had the lowest flavonoid (0.33 mg/100g), total phenol (975.87 mg/100g), iron (2.23 mg/100g) and vitamin C (1.85 mg/100g), but highest values for alkaloid (1.80 mg/100g), Magnesium (142.01 mg/100g) and the quality indices. The fresh P. guineense seeds had the highest tannins (3.55 mg/100g) and the B-vitamins, while the dried P. guineense leaves had the highest iron (3.14 mg/100g) and the lowest tannins (3.23 mg/100g). The seeds had significant amounts of crude fat. The study concluded that fresh P. guineense leaves had the highest nutrient quality, making them a valuable resource for food and medicine. The study’s findings have implications for the preservation and utilization of P. guineense, particularly in tropical regions where the plant is widely used. The study examined the effect of drying on the leaves and seeds of P. guineense, providing insights into the impact of drying on the properties of these two consumed plant parts.

 

References

Adejumo, O. E., Kolapo, A. L., & Farolarin, A. O. (2012). Moringa oleifera Lam. (Moringaceae) grown in Nigeria: In vitro antisickling activity on deoxygenated erythrocyte cells. Journal of Pharmacy and Bioallied Sciences, 4(2), 118–122. https://doi.org/10.4103/0975-7406.94812

Ademuyiwa, O. H., Fasogbon, B. M., & Adebo, O. A. (2023). The potential role of Piper guineense (black pepper) in managing geriatric brain aging: A review. Critical Reviews in Food Science and Nutrition, 63(16), 2840–2850. https://doi.org/10.1080/10408398.2021.1980764

Adum, U. U., & Inyang, U. E. (2024). Optimization of drying parameters of hot leaves (Piper guineense) using artificial neural network and response surface methodology. Journal of Systematic and Modern Science Research, 4(9), 29–44.

Agbaire, P. O. (2012). Quality assessment of palm oil sold in some major markets in Delta State, southern Nigeria. African Journal of Food Science and Technology, 3(9), 223–226.

Ajayi, O. B., Akomolafe, S. F., & Adefioye, A. (2014). Proximate analysis, mineral contents, amino acid composition, anti-nutrients and phytochemical screening of Brachystegia eurycoma Harms and Piper guineense Schum and Thonn. American Journal of Food and Nutrition, 2(1), 11–17. https://doi.org/10.12691/ajfn-2-1-3

Aké, C. B., Ta, B. I. H., & Koné, M. W. (2019). Some minerals of nutritional and therapeutic importance from the leaves and stems of Piper guineense Schum. & Thonn. (Piperaceae). GSC Biological and Pharmaceutical Sciences, 8(3), 104–108. https://doi.org/10.30574/gscbps.2019.8.3.0154

Alademeyin, J. O., & Arawande, J. O. (2016). Effect of processing on physicochemical properties and fatty acid composition of fluted pumpkin (Telfairia occidentalis) seed oil. Pakistan Journal of Scientific and Industrial Research Series A: Physical Sciences, 59(2), 83–89. https://doi.org/10.52763/PJSIR.PHYS.SCI.59.2.2016.83.89

Alagbe, O. A., Alagbe, G. O., Adekunle, E. A., Ayodele, O. O., Olorode, E. M., Oyediran, R. I., Oloyede, E. O., & Oyeleye, A. O. (2021). Ethnomedicinal uses and therapeutic activities of Piper guineense: A review. Journal of Applied Sciences and Environmental Management, 25(6), 927–937. https://doi.org/10.4314/jasem.v25i6.6

Amaechi, N. C., Ihechere, L. A., & Isaac, P. U. (2020). Nutrient composition, color, rehydration ratio and microstructure of Moringa oleifera leaves: The effect of various drying methods. Annals. Food Science and Technology, 21(3), 442–450.

Amin, M. Z., Islam, T., Mostofa, F., Uddin, M. J., Rahman, M. M., & Satter, M. A. (2019). Comparative assessment of the physicochemical and biochemical properties of native and hybrid varieties of pumpkin seed and seed oil (Cucurbita maxima Linn). Heliyon, 5(12), Article e02994. https://doi.org/10.1016/j.heliyon.2019.e02994

Andina, L., Riyanto, R., & Rohman, A. (2014). Determination of anisidine value of red fruit oil under elevated temperature using FTIR spectroscopy and multivariate calibration. International Food Research Journal, 21(6), 2325–2330.

Anjorin, T. S., Ikokoh, P., & Okolo, S. (2010). Mineral composition of Moringa oleifera leaves, pods and seeds from two regions in Abuja, Nigeria. International Journal of Agriculture & Biology, 12(3), 431–434.

AOAC International. (2000). Official methods of analysis of AOAC International (17th ed.).

AOAC International. (2016). Official methods of analysis of AOAC International (20th ed.).

Arowora, K. A., Ezeonu, C. S., Imo, C., & Nkaa, C. G. (2017). Protein levels and amino acids composition in some leaf vegetables sold at Wukari in Taraba State, Nigeria. International Journal of Biological Sciences and Applications, 4(2), 19–24.

Attaugwu, R. N., & Uvere, P. O. (2017). Health-promoting properties of Alternanthera brasiliana leaves and Hibiscus sabdariffa calyces used in fortification of maize-bambara groundnut malt and maize-cowpea malt complementary foods. Food Research, 1(4), 133–139. https://doi.org/10.26656/fr.2017.4.058

Bonnie, T., & Choo, Y. M. (2000). Valuable minor constituents of commercial red palm olein: Carotenoids, vitamin E, ubiquinones and sterols. Journal of Oil Palm Research, 12(1), 14–24.

Carson, N., Tumilaar, S. G., Kurnia, D., Latipudin, D., & Satari, M. H. (2022). A review of bioactive compounds and antioxidant activity properties of Piper species. Molecules, 27(19), 6774. https://doi.org/10.3390/molecules27196774

Chandran, R. P., Manju, S., Vysakhi, M. V., Shaji, P. K., & Achuthan Nair, G. (2013). In vitro antioxidant potential of methanolic and aqueous extracts of Ardisia solanacea Roxb. leaf. Journal of Pharmacy Research, 6(5), 555–558. https://doi.org/10.1016/j.jopr.2013.04.038

Chikwendu, J. N., & Nmalubuikeibe, F. (2014). Nutrient and phytochemical composition and effects of boiling on Amaranthus hybridus seeds. International Journal of Science and Technolodge, 2(12), 163–168.

Chinwendu, S., Ejike, E. N., Ejike, B. U., Oti, W., & Nwachukwu, I. (2016). Phytochemical properties of uziza leave (Piper guineense). European Journal of Pure and Applied Chemistry, 3(2), 12–15.

Chun, J., Lee, J., Ye, L., Exler, J., & Eitenmiller, R. R. (2006). Tocopherol and tocotrienol contents of raw and processed fruits and vegetables in the United States diet. Journal of Food Composition and Analysis, 19(2-3), 196–204. https://doi.org/10.1016/j.jfca.2005.08.001

de Sá, M. C., & Rodriguez-Amaya, D. B. (2004). Optimization of HPLC quantification of carotenoids in cooked green vegetables: Comparison of analytical and calculated data. Journal of Food Composition and Analysis, 17, 37–51. https://doi.org/10.1016/S0889-1575(03)00100-5

Dibulo, C. C., Madu, K. C., Ogbu, P. N., Onyeachu, B. I., & Njoku, D. I. (2017). Proximate and phytochemical analysis of ethanolic extracts of leaves of Piper guineese from South-eastern Nigeria. IOSR Journal of Applied Chemistry, 10(8), 46–50.

Dingtsen, D. J., Bartholomew, O., John, S. S., Eyitayo, E. A., & Ekirigwe, O. (2020). Evaluation of the antinutritional and nutritional composition of five Nigerian spices. American Journal of Quantum Chemistry and Molecular Spectroscopy, 5(1), 22–27. https://doi.org/10.11648/j.ajqcms.20200402.12

D'Mello, J. P. F. (Ed.). (2003). Food safety: Contaminants and toxins. CABI Publishing.

Domínguez, R., Gómez, M., Fonseca, S., & Lorenzo, J. M. (2014). Effects of different cooking methods on lipid oxidation and formation of volatile compounds in foal meat. Meat Science, 97(2), 223–230. https://doi.org/10.1016/j.meatsci.2014.01.023

Edeoga, H. O., Omosun, G., & Uche, L. C. (2006). Chemical composition of Hyptis suaveolens and Ocimum gratissimum hybrids from Nigeria. African Journal of Biotechnology, 5(10), 892–895.

Eitenmiller, R. R., & Lee, J. (2004). Vitamin E: Food chemistry, composition, and analysis. CRC Press. https://doi.org/10.1201/9780203970140

Ekeanyanwu, C. R., Ogu, G. I., & Nwachukwu, P. U. (2010). Biochemical characteristics of the African nutmeg (Monodora myristica). Agricultural Journal, 5(5), 303–308. https://doi.org/10.3923/aj.2010.303.308

EL-Qudah, J. M. (2009). Identification and quantification of major carotenoids in some vegetables. American Journal of Applied Sciences, 6(3), 492–497. https://doi.org/10.3844/ajassp.2009.492.497

Emeziem, D. M., & Iwu, I. C. (2024). GC-MS characterization of phytochemicals and anti-microbial properties of Chromolaena odorata leaf harvested from South Eastern Nigeria. Asian Journal of Biochemistry, Genetics and Molecular Biology, 16(7), 85–97. https://doi.org/10.9734/ajbgmb/2024/v16i7393

Enyi, C. U., & Ojimelukwe, P. C. (2021). Processing methods and storage period affect the quality of palm oil. Carpathian Journal of Food Science and Technology, 13(4), 192–210. https://doi.org/10.34302/crpjfst/2021.13.4.15

Enyi, C. U., Ojimelukwe, P. C., & Ugwuona, F. U. (2025). Comparative study on the yield, amino acid profile and cowhide (ponmo) tenderization efficiency of papain from different parts of papaya (Carica papaya). Records of Agricultural and Food Chemistry, 5(1), 10–20. https://doi.org/10.25135/rfac.26.2501.3398

Evuen, U. F., Okolie, N. P., & Apiamu, A. (2022). Evaluation of the mineral composition, phytochemical and proximate constituents of three culinary spices in Nigeria: A comparative study. Scientific Reports, 12, Article 21117. https://doi.org/10.1038/s41598-022-25204-3

Ezenobi, U. V., Amaku, F. J., & Agbidi, C. (2016). Phytochemical, proximate, minerals and vitamin composition of Monodora myristica and Piper guineese seeds. Research Journal of Science and Technology, 8(4), 209–214. https://doi.org/10.5958/2349-2988.2016.00031.0

Ghosh, D. (2015). Tannins from foods to combat diseases. International Journal of Pharma Research & Review, 4(5), 40–44.

Gimeno, E., Calero, E., Castellote, A. I., Lamuela-Raventós, R. M., De la Torre, M. C., & López-Sabater, M. C. (2000). Simultaneous determination of α-tocopherol and β-carotene in olive oil by reversed-phase high-performance liquid chromatography. Journal of Chromatography A, 881(1-2), 255–259. https://doi.org/10.1016/S0021-9673(00)00272-7

Guillén, M. D., & Cabo, N. (2002). Fourier transform infrared spectra data versus peroxide and anisidine values to determine oxidative stability of edible oils. Food Chemistry, 77(4), 503–510. https://doi.org/10.1016/S0308-8146(01)00371-5

Harborne, J. B. (1998). Phytochemical methods: A guide to modern techniques of plant analysis (3rd ed.). Springer Science & Business Media.

Hidalgo, A., Brandolini, A., & Pompei, C. (2009). Kinetics of tocols degradation during the storage of einkorn (Triticum monococcum L. ssp. monococcum) and bread wheat (Triticum aestivum L. ssp. aestivum) flours. Food Chemistry, 116(4), 821–827. https://doi.org/10.1016/j.foodchem.2009.01.075

Hiol, A., Comeau, L. C., Druet, E., Jonzo, M. D., Rugani, N., & Sarda, L. (2000). Purification and characterization of an extracellular lipase from a thermophilic Rhizopus oryzae strain isolated from palm fruit. Enzyme and Microbial Technology, 26(5-6), 421–430. https://doi.org/10.1016/S0141-0229(99)00173-8

Imo, C., Uhegbo, F. O., Ifeanacho, N. G., & Azubuike, N. C. (2015). Histological and hepatoprotective effect of Gongronema latifolium Benth in acetaminophen-induced hepatic toxicity in male albino rats. International Journal of Preventive Medicine Research, 1(4), 217–226.

Imo, C., Yakubu, O. E., Imo, N. G., Udegbunam, I. S., Tatah, S. V., & Onukwugha, O. J. (2018). Proximate, mineral and phytochemical composition of Piper guineense seeds and leaves. Journal of Biological Sciences, 18(7), 329–337. https://doi.org/10.3923/jbs.2018.329.337

Iwu, I. C., Onu, U., & Ukaoma, A. A. (2019). Characterization of the volatile components of the leaf of Stachytarpheta cayennensis (Rich.) Vahl. International Journal of Herbs, Spices and Medicinal Plants, 4(1), 41–49.

Kabiru, Y. A., Okolie, N. L., Muhammad, H. L., & Ogbadoyi, E. O. (2012). Preliminary studies on the antiplasmodial potential of aqueous and methanol extracts of Eucalyptus camaldulensis leaf. Asian Pacific Journal of Tropical Disease, 2(Suppl. 2), S809–S814. https://doi.org/10.1016/S2222-1808(12)60270-9

Kimura, M., & Rodriguez-Amaya, D. B. (2002). A scheme for obtaining standards and HPLC quantification of leafy vegetable carotenoids. Food Chemistry, 78(3), 389–398. https://doi.org/10.1016/S0308-8146(02)00203-0

Kirk, R. S., & Sawyer, R. (1991). Pearson's composition and analysis of foods (9th ed.). Longman Scientific & Technical.

Kopsell, D. A., & Kopsell, D. E. (2006). Accumulation and bioavailability of dietary carotenoids in vegetable crops. Trends in Plant Science, 11(10), 499–507. https://doi.org/10.1016/j.tplants.2006.08.006

Meda, A., & Lamien, C. E. (2005). Determination of the total phenolic, flavonoid and proline contents in Burkina Fasan honey, as well as their radical scavenging activity. Food Chemistry, 91(3), 571–577. https://doi.org/10.1016/j.foodchem.2004.10.006

Mgbeahuruike, E. E., Fyhrquist, P., Vuorela, H., Julkunen-Tiitto, R., & Holm, Y. (2018). Alkaloid-rich crude extracts, fractions and piperamide alkaloids of Piper guineense possess promising antibacterial effects. Antibiotics, 7(4), Article 98. https://doi.org/10.3390/antibiotics7040098

Morris, A., Barnett, A., & Burrows, O. (2004). Effect of processing on nutrient content of foods. Cajanus, 37(3), 160–164.

Müller, J., & Heindl, A. (2006). Drying of medicinal plants. In R. J. Bogers, L. E. Craker, & D. Lange (Eds.), Medicinal and aromatic plants: Agricultural, commercial, ecological, legal, pharmacological and social aspects (pp. 237–252). Springer.

Núñez, V., Castro, V., Murillo, R., Ponce-Soto, L. A., Merfort, I., & Lomonte, B. (2005). Inhibitory effects of Piper umbellatum and Piper peltatum extracts towards myotoxic phospholipases A2 from Bothrops snake venoms: Isolation of 4-nerolidylcatechol as active principle. Phytochemistry, 66(9), 1017–1025. https://doi.org/10.1016/j.phytochem.2005.03.026

Nwachukwu, C. U., Ume, N. C., Obasi, M. N., Nzewuihe, G. U., & Onyirioha, C. A. (2010). The qualitative uses of some medicinal plants in Ikeduru LGA of Imo State, Nigeria. New York Science Journal, 3(11), 129–134.

Ogbedeagu, C. O., Anozie, G. O., Enyi, C. U., & Nwachukwu, C. A. (2025a). Proximate, phytochemical and sensory evaluations of African black pepper (Piper guineense), Guinea pepper (Xylopia aethiopica) and aidan fruit (Tetrapleura tetraptera) spiced drinks. Bioorganic and Medicinal Chemistry Reports, 8(2), 54–66.

Ogbedeagu, C. O., Enyi, C. U., & Nwachukwu, C. A. (2025b). Nutrient composition and techno-functional properties of three Nigerian spices and sensory acceptability of traditional dishes. Records of Agricultural and Food Chemistry, 5(2), 180–192. https://doi.org/10.25135/rfac.42.2511.3740

Ogbonna, A. C., Abuajah, C. I., & Hart, E. B. (2015). Preliminary evaluation of physical and chemical properties of Piper guineense and Xylopia aethiopica seed oils. International Food Research Journal, 22(4), 1404–1409.

Ogbuji, C. A., Ndulaka, J. C., & David-Chukwu, N. P. (2016). Comparative evaluation of mineral compositions of green leafy vegetables consumed in South Eastern Nigeria. African Journal of Food Science, 10(12), 374–378.

Ogunka-Nnoka, C. U., Igwe, F. U., & Orubite, K. O. (2015). Comparative study of the physicochemical properties and fatty acid composition of some indigenous spices in Nigeria. International Journal of Biochemistry Research & Review, 5(2), 171–177. https://doi.org/10.9734/IJBCRR/2015/13305

Ogunmefun, O. T., Akharaiyi, F. C., & Adegunle, S. J. (2017). Phytochemical and antimicrobial properties of Piper guineense (Schumach. & Thonn.) on selected human pathogens. Journal of Chemical and Pharmaceutical Research, 9(11), 180–186.

Ojimelukwe, P. C. (2023). Piper guineense—An underutilized aromatic spice with medicinal value. Advances in Traditional Medicine, 23(2), 381–392. https://doi.org/10.1007/s13596-021-00586-3

Ojinnaka, M. C., Odimegwu, E. N., & Chidiebere, F. E. (2016). Comparative study on the nutrient and antinutrient composition of the seeds and leaves of uziza (Piper guineense). IOSR Journal of Environmental Science, Toxicology and Food Technology, 10(8), 42–48. https://doi.org/10.9790/2402-1008014248

Okafor, J. C. (1983). Horticulturally promising indigenous wild plant species of the Nigerian forest zone. Acta Horticulture, 123, 165–176. https://doi.org/10.17660/ActaHortic.1983.123.15

Okeke, C. U., & Elekwa, I. (2003). Phytochemical study of the extract of Gongronema latifolium Benth. Journal of Health and Visual Sciences, 5(1), 47–55.

Okwu, D. E., & Josiah, C. (2006). Evaluation of the chemical composition of two Nigerian medicinal plants. African Journal of Biotechnology, 5(4), 357–361.

Omorodion, T. N., Wasswa, J. N., Achukwu, P. U., & Osamede, A. S. (2017). Histopathological effect of crude extract of Piper guineense (uziza) leaves on the liver of Wistar rats and on pseudofolliculitis barbae (after shave rash) in human. American Journal of Biomedical Sciences, 9(2), 85–90. https://doi.org/10.5099/aj170200085

Onwuka, G. I. (2018). Food analysis and instrumentation: Theory and practice (2nd ed.). Naphtali Prints.

Onwuka, M. N., Nwosu, C. N., & Okerulu, A. L. (2022). Pharmacutive evidence of Piper guineense on reproductive toxicity. The Journal of Phytopharmacology, 11(3), 221–223. https://doi.org/10.31254/phyto.2022.11315

Onyeka, O., Nwoye, B., Anya, O. U., & Ezejiofor, C. C. (2018). Analysis of the proximate and mineral contents of uziza (Piper guineense) seeds from three different states (Ebonyi, Cross-River and Delta) in the southern part of Nigeria. International Journal of Food Science and Nutrition Engineering, 8(1), 10–14.

Ouilly, J. T., Bazongo, P., Bougma, A., Kaboré, N., Lykke, A. M., Ouédraogo, A., & Bassolé, I. H. N. (2017). Chemical composition, physicochemical characteristics, and nutritional value of Lannea kerstingii seeds and seed oil. Journal of Analytical Methods in Chemistry, 2017, Article 2840718. https://doi.org/10.1155/2017/2840718

Oyemitan, I. A., Kolawole, F., & Oyedeji, A. O. (2014). Acute toxicity, antinociceptive and anti-inflammatory activity of the essential oil of fresh fruits of Piper guineense Schum Thonn (Piperaceae) in rodents. Journal of Medicinal Plants Research, 8(40), 1191–1197.

Prakash, R., Gandotra, S., Singh, L. K., Das, B., & Lakra, A. (2008). Rapid resolution of delusional parasitosis in pellagra with niacin augmentation therapy. General Hospital Psychiatry, 30(6), 581–584. https://doi.org/10.1016/j.genhosppsych.2008.04.011

Ramadass, S., Basu, S., & Srinivasan, A. R. (2015). Serum magnesium levels as an indicator of status of diabetes mellitus type 2. Diabetes & Metabolic Syndrome: Clinical Research & Reviews, 9(1), 42–45. https://doi.org/10.1016/j.dsx.2014.02.024

Sahabi, D. M., Shehu, R. A., Saidu, Y., & Abdullahi, A. S. (2012). Screening for total carotenoids and β-carotene in some widely consumed vegetables in Nigeria. Nigerian Journal of Basic and Applied Sciences, 20(3), 225–227.

Sebastian, A., Ghazani, S. M., & Marangoni, A. G. (2014). Quality and safety of frying oils used in restaurants. Food Research International, 64, 420–423. https://doi.org/10.1016/j.foodres.2014.07.033

Singleton, V. L., Orthofer, R., & Lamuela-Raventos, R. M. (1999). Analysis of total plant phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteu reagent. Methods in Enzymology, 299, 152–178. https://doi.org/10.1016/S0076-6879(99)99017-1

Stafford, G. I., Jäger, A. K., & van Staden, J. (2005). Effect of storage on the chemical composition and biological activity of several popular South African medicinal plants. Journal of Ethnopharmacology, 97, 107–115. https://doi.org/10.1016/j.jep.2004.10.021

Trang, H. K. (2013). Development of HPLC methods for the determination of water-soluble vitamins in pharmaceuticals and fortified food products [Master's thesis, Clemson University]. TigerPrints. https://open.clemson.edu/all_theses/1745

Umedum, N. L., Udeozo, I. P., Muoneme, O., Okoye, N., & Iloamaeke, I. (2013). Proximate analysis and mineral content of three commonly used seasonings in Nigeria. IOSR Journal of Environmental Science, Toxicology and Food Technology, 5(1), 11–14. https://doi.org/10.9790/2402-0511114

Uzoekwe, N. M., & Ezenwajiugo, C. E. (2023). Phytochemicals, elemental and proximate analyses of Piper guineense leaves. Journal of Applied Sciences and Environmental Management, 27(4), 657–663. https://doi.org/10.4314/jasem.v27i4.3

Verma, D. K., & Srivastav, P. P. (2017). Proximate composition, mineral content, and fatty acids analyses of aromatic and non-aromatic Indian rice. Rice Science, 24(1), 21–31. https://doi.org/10.1016/j.rsci.2016.05.005

Yoshida, Y., Niki, E., & Noguchi, N. (2003). Comparative study on the action of tocopherols and tocotrienols as antioxidant: Chemical and physical effects. Chemistry and Physics of Lipids, 123(1), 63–75. https://doi.org/10.1016/S0009-3084(02)00164-0

Downloads

Published

04-09-2026

How to Cite

Uchenna Enyi, C., C. Ojimelukwe, P., Okorie-Humphrey, C., Obumneme Enyi, O., Obiageri Ogbedeagu, C., & Norbert Eze, C. (2026). Effects of heat treatment on the nutrient composition, phytochemical properties and indices of fat quality of fresh and dried leaves and seeds of Piper guineense (Uziza). Food Agricultural Sciences and Technology, 12(3), 208–235. retrieved from https://ph02.tci-thaijo.org/index.php/stej/article/view/262010