การสะสม การเคลื่อนย้าย และผลกระทบของอนุภาคเงินนาโนที่มีต่อข้าวหอมมะลิ 105 ข้าวเหนียว กข 6 และผักบุ้งจีน (ACCUMULATION, TRANSLOCATION AND IMPACTS OF SILVER NANOPARTICLES ON RICE (ORYZA SATIVA L.CV. KDML105), STICKY RICE (ORYZA SATIVA VAR. GLUTINOSA CV. RD 6) AND CHINESE WATER CONVOLVULUS (IPOMOEA AQUATICA Forsk. VAR. REPTAN))

Abstract

            Currently, silver nanoparticles (AgNPs) are widely used in agriculture area due to their properties as anti-bacterial and fungi. AgNPs may be released into the environment and impacts on agricultural crops have been exposed. In this work, to investigate the effects of AgNPs, accumulate, translocation and impacts on three plants including rice (Oryza sativa L. cv. KDML 105), sticky rice (Oryza sativa var. glutinosa cv. RD 6) and Chinese water convolvulus (Ipomoea aquatic Forsk. Var. reptan) were studied. The AgNPs were synthesized by pure natural honey as a reducing agent. In addition, the wet digestion of D1, D2 and D3 methods for determination of silver accumulation were evaluated. The three plants were exposed to AgNPs with various concentrations of 0.02, 0.05, 0.1 and 1 mg/L. After wet digestion the silver concentration were determined by graphite furnace atomic absorption spectroscopy (GFAAS). The results showed that the D2 method was the optimum wet digestion process for the determination of silver in plants with the percent recovery of 81.67 to 94.00. The aggregation and accumulation of AgNPs within the roots and shoots of three plants were found that the roots of sticky rice at a concentration of 0.05 mg/L and penetrated to the cell wall and cell in root leading to effects on structural features and transporting nutrients to the plants, including the development on the growth of plant cells