ผลของการปรับสภาพด้วยด่างและการลดความเป็นโซ่กิ่งด้วยกรด ในแกลบ ฟาง และกากรำข้าวสกัดน้ำมัน ต่อการผลิตไซโลไบโอสด้วยเอนไซม์ไซแลนเนสทางการค้า THE EFFECT OF ALKALI PRETREATMENT AND ACID DEBRANCHING ON RICE HUSK, RICE STRAW, AND DEFATTED RICE BRAN FOR XYLOBIO()
คำสำคัญ:
Biomass, Alkali Pretreatment, Acid Debranching, Endoxylanase, Xylobioseบทคัดย่อ
วัสดุชีวมวล The use of alkali pretreatment and acid debranching to optimize the arabinose/xylose ratio of arabinoxylan from rice straw (RS), rice husk (RH), and defatted rice bran (DRB) as preparation for production of xylobiose and/or arabino-xylooligosacharides using commercial xylanases were investigated. Firstly, each biomass was treated with alkali pretreatment with 2% NaOH and acid debranching with formic acid. The yields of RH, RS, and DRB after alkali pretreatment appeared to be 68.7, 44.8, and 24.3% while the total sugar contents were increased from 19.9 to 28.0%, 21.7 to 26.4%, and 11.3 to 20.0%, compared to untreated biomass, respectively. The total sugar content of acid debranched biomass was also increased up to 4%. In addition, the alkali pretreatment slightly decreased arabinose/xylose ratio (A/X) of DRB, RH, and RS from 1.08 to 0.82, 0.14 to 0.13 and 0.22 to 0.22. At the same time, arabinoxylan contents were increased from 5.8 to 14.3%, 11.5 to 18.6% and 11.0 to 18.3%, respectively. Superior to alkali pretreatment, the following acid debranching dramatically decreased A/X ratio of DRB from 1.08 to 0.63. After mentioned processes, xylobiose was separately produced by using Pentopan Mono BG and Pentopan 500 BG at 132.5 U and 26.5 U/g of substrate, respectively. The hydrolysis was performed in sodium phosphate buffer (pH 6.0) at 50°C for 4 h with continuously shaking at 150 rpm. The results showed that the biomass with higher A/X ratio was lesser hydrolysed. Pentopan 500 BG exhibited greater hydrolysis effect than that of Pentopan Mono BG. However, the Thin layer chromatography and Ion chromatography results confirmed that the hydrolysates from both xylanases had the similar sugar patterns showing xylose, arabinose, xylobiose, and the components with a higher degree of polymerization. However, Pentopan 500 BG remarkably produced monosaccharides mainly xylose and arabinose. The alkali pretreatment singly was enough while from DRB, the acid debranching should be combined following the pretreatment process.
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