ผลของการใช้โอโซนร่วมกับยูวี-ซีหรือละอองลอยจากสารละลายไฮโดรเจนเปอร์ออกไซด์ในการกำจัดเชื้อเอสเชอริเชียโคไลและเอสเปอร์จิลลัสไนเจอร์ที่ปนเปื้อนในโรงงานอุตสาหกรรมก๋วยเตี๋ยว (EFFECTS OF COMBINED OZONATION AND UV-C TREATMENT OR VAPORED HYDROGEN PEROXIDE FUMIGATION TO INACTIVATE ESCHERICHIA COLI AND ASPERJILLUS NIGER CONTAMINANTS IN RICE NOODLE INDUSTRY)
คำสำคัญ:
Combined Ozonation and UV-C Treatment, Vaporized Hydrogen Peroxide, Inactivate, Escherichia coli Aspergillus nigerบทคัดย่อ
The ability of ozonation and UV-C applied to eradicate total plate count, yeast/mold and Escherichia coli contamination were tested on a flour slurry and to study the application of vapored hydrogen peroxide (VHP) for sanitizing the chamber.
Method: A prototype system, consisting of ozone generator (2 L/min) and UV sterilizer (45 W), was fabricated and tested on flour slurry systems using the same initial E. coli and A. niger concentration. In this experiment, the ratios of flour slurry to water were prepared at 0, 20, 45, 75 and 100%. The flour slurry (15 L) were spiked with E. coli or A. niger at 7 log CFU/mL approximately 200 mL. The circulation mass flow rates were fixed at 0.3 kg/s. Sampling time intervals were 0, 5, 10, 20, 30 and 40 min. Total plate count (TPC), yeast/mold and E. coli was achieved by taking 1 mL aliquot of sample.
Result: The result showed the destruction profiles of TPC, yeast/mold and E. coli counts as a function of time and ratio of flour slurry. After Ozone and UV treatments for 40 min, the TPC, yeast/mold and E. coli counts were reduced to 0 log CFU/mL in the 20% of flour slurry. For the flour slurry concentrations higher than 20%, the ozone with UV treatment was able to bring down the microbial counts to only 3 - 2 log reductions, respectively. However, the ozone with the UV treatment was not able to reduce TPC, yeast/mold and E. coli counts at the 100% of flour slurry treatment because it has high solid loading, turbidity and viscosity. In all treatments with 1-5% hydrogen peroxide (H2O2), there was an instant drop of E. coli numbers within the first 2 min from 109 to 105 CFU/mL or 4 log reductions. It was shown that even at shorter time periods could eliminate organisms as long as H2O2 was well distributed and maintained at an effective concentration to destroy E. coli cells. The number of the fungal colonies decreased when the concentration of VHP were increased. When the addition of H2O2 at 3 and 5% concentrations at exposure times of 4-6 min produced incremental increases in A. niger destruction.
Conclusion: The combined ozone/UV treatments have been shown to be more appropriate treatments for cloudy and turbid medium, like flour slurry. The use of combined treatment was able to achieve zero E. coli and A. niger count. For fumigation with VHP, we found that VHP decontamination reduce bacterial and fungal contamination in chamber. H2O2 is easily evaporated or destroyed after use (readily decomposing into water and oxygen), has no unpleasant lingering odor, and poses minimal safety problems for workers if handled properly.
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[2] Ahmed, I.; Qazi, I.; and Ullah, J. (2016). Rice Noodles: Materials, Processing and Quality Evaluation. B. Life and Environmental Sciences. 53(3): 215–238.
[3] Cho, M.; Chung, H.; and Yoon, J. (2002). Effect of pH and Importance of Ozone Initiated Radical Reactions in Inactivating Bacillus subtilis spore. Ozone Science & Engineering. 24: 145–150.
[4] Kim, JG.; Yousef, AE.; and Khadre, MA. (2003). Ozone and Its Current and Future Application in the Food Industry. Advances in Food and Nutrition Research. 45: 167-218.
[5] McMeekin, T.A.; Baranyi, J.; Bowman, J.; Dalgaard, P.; Kirk, M.; Ross, T.; Schmid, S.; and Zwietering, M.H. (2006). Information Systems in Food Safety Management. International Journal of Food Microbiology. 112: 181-194.
[6] Gormley, F.J.; Little, C.L.; de Pinna, E.; and McLauchlin, J. (2010). The Microbiological Safety of Ready-to-Eat Specialty Meats from Markets and Specialty Food Shops: A UK Wide Study with a Focus on Salmonella and Listeria monocytogenes. Food Microbiology. 27: 243-249.
[7] Selma, M.; Allende, A.; Lopez-Galvez, F.; Conesa, M.A.; and Gil, M.I. (2008). Disinfection Potential of Ozone, Ultraviolet-C and their Combination in Wash Water for the Fresh-Cut Vegetable Industry. Food Microbiology. 25: 809-814.
[8] Meunier, L.; Canonica, S.; and Von, Gunten U. (2006). Implications of Sequential Use of UV and Ozone for Drinking Water Quality. Water Research. 40(9): 1864–1876.
[9] Beltran, FJ.; Rivas, FJ.; and Montero, R. (2004) A TiO2/AL2O3 Catalyst to Improve the Ozonation of Oxalic Acid in Water. Applied Catalysis B. 47(2): 101-109.
[10] Barnes, L.M.; Lo, M.F.; Adams, M.R.; and Chamberlain, A.H.L. (1999). Effect of Milk Proteins on Adhesion of Bacteria to Stainless Steel Surfaces. Applied and environmental microbiology. 65(10): 4543-4548.
[11] Giaouris, E.; Heir, E.; Hébraud, M.; Chorianopoulos, N.; Langsrud, S.; Møretrø, T.; and Nychas, G.J. (2014). Attachment and Biofilm Formation by Foodborne Bacteria in Meat Processing Environments: Causes, Implications, Role of Bacterial Interactions and Control by Alternative Novel Methods. Meat Science. 97(3): 298-309.
[12] Bloomfield, S.F. (2003). Home Hygiene: a Risk Approach. International journal of hygiene and environmental health. 206: 1-8.
[13] Erickson, M.C.; Liao, J.; Cannon, J.L.; and Ortega, Y.R. (2015). Contamination of Knives and Graters by Bacterial Foodborne Pathogens During Slicing and Grating of Produce. Food microbiology. 52: 138-145.
[14] Price, D.L.; Morris, B.N.; and Lagrange, G. (2007, July ). Assessment of Accelerated Hydrogen Peroxide for Sanitizing Carpet. In The Annual Meeting and Exhibition 2007.
[15] Noonimabe, P.; Mahakarnchanakul, W.; Nielsen, K.F.; Frisvad, J.C.; and Samson, R.A. (2009). Fumonisin B2 Production by Aspergillus niger in Thai Coffee Beans. Food Additives and Contaminants. 26: 94-100.
[16] Al-Abdalall, A.H.A. (2009). Production of Aflatoxins by Aspergillus flavus and Aspergillus niger strains Isolated from Seeds of Pulses. Journal of Food, Agriculture and Environment. 7(2): 33-39.
[17] Rutala, W.A.; and Weber, D.J. (2010). Guideline for Disinfection and Sterilization of Prion-Contaminated Medical Instruments. Infection Control & Hospital Epidemiology. 31(2): 107-117.
[18] Kimura, T. (2012). Effective Decontamination of Laboratory Animal Rooms with Vapour-Phase (†œVaporizedâ€) Hydrogen Peroxide and Peracetic Acid. Scandinavian Journal of Laboratory Animal Sciences. 39(1): 17-23.
[19] Taneja, N.; Biswal, M.; Kumar, A.; Edwin, A.; Sunita, T.; Emmanuel, R.; Gupta, A.K.; and Sharma, M. (2011). Hydrogen Peroxide Vapour for Decontaminating Air-Conditioning Ducts and Rooms of an Emergency Complex in Northern India: Time to Move on. Journal of Hospital Infection. 78(3): 200-203.
[20] Cheng, M.; Zeng, G.; Huang, D.; Lai, C.; Xu, P.; Zhang, C.; and Liu, Y. (2016). Hydroxyl Radicals based
Advanced Oxidation Processes (AOPs) for Remediation of Soils Contaminated with Organic Compounds: a review. Chemical Engineering Journal. 284: 582-598.
[21] McDonnell, G.; Bonfield, P.; and Hernandez, V.D. (2007). The Safe and Effective Fumigation of Hospital Areas with a New Fumigation Method based on Vaporized Hydrogen Peroxide. American Journal of Infection Control. 35(5): E33-E34.
[22] Herdt, J.; and Feng, H. (2009). Aqueous Antimicrobial Treatments to Improve Fresh and Fresh-cut Produce Safety. Microbial safety of fresh produce. Wiley-Blackwell. Ames. IA: 169-190.
[23] Jiang, Y.; Fan, X.; Li, X.; Gurtler, J.B.; Mukhopadhyay, S.; and Jin, T. (2017). Inactivation of Salmonella Typhimurium and Quality Preservation of Cherry Tomatoes by in-package Aerosolization of Antimicrobials. Food Control. 73: 411-420.
[24] Luukkonen, T.; Teeriniemi, J.; Prokkola, H.; Rämö, J.; and Lassi, U. (2014). Chemical Aspects of Peracetic Acid based Wastewater Disinfection. Water SA. 40(1): 73-80.
[25] Karabulut, O.A.; Ilhan, K.; Arslan, U.; and Vardar, C. (2009). Evaluation of the Use of Chlorine Dioxide by Fogging for Decreasing Postharvest Decay of fig. Postharvest biology and technology. 52(3), 313-315.
[26] Khueankhancharoen, J.; and Thipayarat, A. (2011). Application of Modified Drop Plate Technique (MDPT) and Logistic Model to Optimize Non-selective Substrates for Salmonella Typhi Resuscitation. Asian Journal of Food and Agro-Industry. 4(6): 349-358.
[27] Prado, J.; and Esplugas, S. (1999). Comparison of Different AOPs Involving Ozone to Eliminate Atrazine. Ozone Science & Engineering. 21(1): 39-52.
[28] Guittoneau, S.; De Laat, J.; Duguet, J.P.; Bonnel, C.; and Dorle, M. (1990). Oxidation of Parachloronitrobenzene in Dilute Aqueous Solution by O3+UV and H2O2+UV: a comparative study‖. Ozone Science and Engineering. 12: 73–94.
[29] Kumar, C.G.; and Anand, S.K. (1998). Significance of Microbial Biofilms in Food Industry: a review. International journal of food microbiology. 42: 9-27.
[30] Glaze, WH.; Kang, JW.; and Chapin, DH. (1987). The Chemistry of Water Treatment Processes Involving Ozone, Hydrogen Peroxide and Ultraviolet Radiation. Ozone: Science & Engineering. 9: 335–342.
[31] Peyton, GR.; and Glaze, WH. (1988). Destruction of Pollutants in Water with Ozone in Combination with Ultraviolet Radiation. 3. Photolysis of Aqueous Ozone. Environmental Science & Technology. 22: 761–769.
[32] Andreozzi, R.; Caprio, V.; Insola, A.; and Marotta, R. (1999). Advanced Oxidation Processes (AOP) for Water Purification and Recovery. Catalysis today. 53: 51-59.
[33] Klapes, NA.; and Vesley, D. (1990). Vapor-phase Hydrogen Peroxide as a Surface Decontaminant and Sterilant. Applied and Environmental Microbiology. 56: 503-506.
[34] McDonnell, G.; and Russell, A.D. (1999). Antiseptics and Disinfectants: Activity, Action, and Resistance. Clinical Microbiology Reviews. 12(1), 147-179.
[35] Sweeney, M.J.; and Dobson, A.D. (1998). Mycotoxin Production by Aspergillus, Fusarium and Penicillium Species. International journal of food microbiology. 43(3): 141-158.
[36] Heckert, RA.; Best, M.; ordan, LT.; Dulac, JGC.; Eddington, DL.; and Sterritt, WG. (1997). Efficacy of Vaporized Hydrogen Peroxide Against Exotic Animal Viruses. Applied and Environmental Microbiology. 63: 3916- 3918.
[37] Kahnert, A.; Seiler, P.; Stein, M.; Aze, B.; McDonnell, G.; and Kaufmann, SHE. (2005). Decontamination with Vaporized Hydrogen Peroxide is Effective Against Mycobacterium tuberculosis. Journal of Applied Microbiology. 40: 448-452.
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