Effects of Immobilized Plant Chlorophyll Cells on Dissolved Oxygen Contents in Synthetic Wastewater
Keywords:
cell model, chloroplast extract, dissolved oxygen, synthetic wastewaterAbstract
This research aimed to investigate the effects of the immobilized plant chlorophyll cells on dissolved oxygen (DO) contents in synthetic wastewater. Plant chlorophyll crude extracts were prepared using three plant leaves: betel (Piper sarmentosum), centella (Centella asiatica), and pandan (Pandanus amaryllifolius). Ten grams of each plant's leaves were homogenized with 100 ml of water. The supernatants were filtrated and mixed with various concentrations of sodium alginate ranging from 0.5, 1.0, 1.5, and 2.0 %(w/v). Each of the chlorophyll-alginate mixtures was added dropwise into various concentrations of calcium chloride solution concentration ranging from 0.5, 1.0, 1.5, and 2.0%(w/v). The optimum concentration for the immobilized plant chlorophyll formation was a combination of 2.0%(w/v) sodium alginate and 1.0%(w/v) calcium chloride. Each immobilized chlorophyll was subjected to determine the DO content in synthetic wastewater using DO meter. The results showed that immobilization with the betel’s chlorophyll gave a maximal DO content (7.51 mg/L in 60 minutes) followed by centella and pandan (6.82 and 5.45 mg/L in 60 minutes, respectively). This finding suggested that the immobilized plant chlorophyll cells could be used for the wastewater treatment process.
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