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The objective of this research was to isolate plant growth promoting rhizobacteria from Wedelia trilobata (L.) A.S. Hitchcock and test their basic properties of plant growth promoters. Forty-seven bacteria isolates were obtained including 26 isolates of gram positive, 18 isolates of gram negative and 3 isolates of actinobacteria. Plant growth promoting properties were tested such as nitrogen fixation, siderophore production, phosphate solubilization and indole-3-acetic acid production. After that 8 isolates were selected for studying of root colonization and plant growth promoting properties including BWTM1_MSU, BWTM2_MSU, BWTM5_MSU, BWTM6_MSU, BWWM6_MSU, BWWM8_MSU, BWWM9_MSU and BWWM16_MSU. The results show that BWTM5_MSU evident promote plant growth with the most seed germination (63.33%), high level of root and shoot length with 6.11 cm and 3.89 cm, respectively. As a result, the root and shoot weight was as high as 9.56 mg and 11.33 mg, respectively. It also correlated with the highest production of indole-3-acetic acid up to 421.29 ug/ml. Seven days after sowing, can isolated BWTM5_MSU from rhizoplane and rhizosphere with 9.67x106 cfu/ml and 1.64x1010 cfu/ml, respectively.
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