Phytochemical and Antioxidant Activity of Thai Traditional Recipe: Akineewakana Recipe
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Abstract
Akineewakana recipe is a Thai traditional medicine that has cannabis as an ingredient which is an elixir medicine group in King Narai the Great. This recipe was used for appetizing, analeptic and antiemesis. These properties are related to the type and quantitative of chemical constituents. Currently, Akineewakana recipe was lack data of phytochemicals. Therefore, this research aims to study the type and phytochemical content by HPLC technique, also the antioxidant activity of Akineewakana recipe was determined. The results showed that the ethanolic Akineewakana recipe extract demonstrated the IC50 value of 56.30±0.29 and 23.39±0.44 µg/ml by DPPH and ABTS assay, respectively. The FRAP value was 448.01±5.72 mmol/100 g Extract. However, this recipe showed lower activity than Trolox (2506.15±13.60 mmol/100 g Extract). The cannabinoid analysis showed a high relative cannabinoid abundance (8130.00±35.59 µg/g Extract) which is d9-THC as the highest content. In addition, it found the relative phenolic and flavonoid abundance as 1804.14±4.86 µg/g Extract. Moreover, the correlation analysis showed that the total phenolic and flavonoid and cannabinoid content correlated with antioxidant activity which is gallic acid and p-hydroxybenzoic acid showed a high correlation with antioxidant significantly. The result suggested that Akineewakana recipe has the potential to be an antioxidant agent. Moreover, this data was used to support the usage of Thai traditional medicine and development of the pharmaceutical products.
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ลิขสิทธ์ ของมหาวิทยาลัยเทคโนโลยีราชมงคลพระนครReferences
M. Sánchez, E. G. Burgos, I. Iglesias, R. Lozano, and M. P. G. Serranillos, “Current uses and knowledge of medicinal plants in the autonomous community of Madrid (Spain): a descriptive cross-sectional study,” BMC Complementary Medicine and Therapies, vol. 20, no. 306, pp. 1-13, Oct. 2020.
N. Lumlerdkij, R. Boonrak, S. Booranasubkajorn, P. Akarasereenont, and M. Heinrich, “In vitro protective effects of plants frequently used traditionally in cancer prevention in Thai traditional medicine: An ethnopharmacological study,” Journal of Ethnopharmacology, vol. 250, pp. 112409, Mar. 2020.
W. Kitdamrongtham, A. Manosroi, H. Akazawa, A. Gidado, P. Stienrut, W. Manosroi, W. Lohcharoenkal, T. Akihisa, and J. Manosroi, “Potent anti-cervical cancer activity: synergistic effects of Thai medicinal plants in recipe N040 selected from the MANOSROI III database,” Journal of Ethnopharmacology, vol. 149, no. 1, pp. 288-296, Aug. 2013.
Department of Thai Traditional and Alternative Medicine, National Thai Traditional Remedies with Ganja, 1st ed. Bangkok: Samchareon Phanich (Bangkok) Co., Ltd. Press, 2021.
A. S. Krzemień, S. Sip, P. Szulc, and J. C. Piontek, “Determining antioxidant activity of cannabis leaves extracts from different varieties-unveiling nature’s treasure trove,” Antioxidants, vol. 12, no. 7, pp. 1390, Jul. 2023.
M. N. Morshed, J. C. Ahn, R. Mathiyalagan, E. J. Rupa, R. Akter, M. R. Karim, D. H. Jung, D.U. Yang, D. C. Yang, and S. K. Jung, “Antioxidant activity of panax ginseng to regulate ROS in various chronic diseases,” Applied Sciences, vol. 13, no. 5, pp. 2893, Feb. 2023.
S. Pagliari, M. Forcella, E. Lonati, G. Sacco, F. Romaniello, P. Rovellini, P. Fusi, P. Palestini, L. Campone, M. Labra, A. Bulbarelli, and I. Bruni, “Antioxidant and anti-inflammatory effect of cinnamon (Cinnamomum verum J. Presl) bark extract after in vitro digestion simulation,” Foods, vol. 12, no. 3, pp. 452, Jan. 2023.
I. Mssillou, A. Agour, A. E. Ghouizi, N. Hamamouch, B. Lyoussi, and E. Derwich, “Chemical composition, antioxidant activity, and antifungal effects of essential oil from Laurus nobilis L. flowers growing in Morocco,” Journal of Food Quality, vol. 2020, pp. 1-8, Dec. 2020.
D. Daorueang, “Antioxidant activity and effect of sa-khan (Piper, Piperaceae) on the normal human proximal tubular epithelial cell line,” Naresuan University Journal: Science and Technology, vol. 28, no. 4, po. 29-35, jun. 2020.
W. Mahavorasirikul, V. Viyanant, W. Chaijaroenkul, A. Itharat, and K. Na-Bangchang, “Cytotoxic activity of Thai medicinal plants against human cholangiocarcinoma, laryngeal and hepatocarcinoma cells in vitro,” BMC Complementary and Alternative Medicine, vol. 28, no. 10, pp. 55, Sep. 2010.
B. Eldhose, V. Notario, and M. S. Latha, “Evaluation of phytochemical constituents and in vitro antioxidant activities of Plumbago indica root extract,” Journal of Pharmacognosy and Phytochemistry, vol. 2, no. 4, pp. 157-161, May. 2013.
S. Kalam, R. Singh, A. Mani, J. Patel, F. N. Khan, and A. Pandey, “Antioxidants: elixir of life,” International Multidisciplinary Research Journal, vol. 2, no.1, pp.18-34, Dec. 2012.
N. Phuyal, P. K. Jha, P. P. Raturi, and S. Rajbhandary, “Total phenolic, flavonoid contents, and antioxidant activities of fruit, seed, and bark extracts of Zanthoxylum armatum DC,” The Scientific World Journal, vol. 2020, pp. 1-7, Mar. 2020.
K. Chaikhong, S. Chumpolphant, P. Rangsinth, C. Sillapachaiyaporn, S. Chuchawankul, T. Tencomnao, and A. Prasansuklab, “Antioxidant and anti-skin aging potential of selected Thai plants: in vitro evaluation and in silico target prediction,” Plants, vol. 12, no. 1, pp. 65, Dec. 2022.
G. J. Molole, A. Gure, and N. Abdissa, “Determination of total phenolic content and antioxidant activity of Commiphora mollis (Oliv.) Engl. Resin,” BMC Chemistry, vol. 16, pp. 48, Jun. 2022.
P. Panyatip, T. Padumanonda, C. Yongram, T. Kasikorn, B. Sungthong, and P. Puthongking, “Impact of tea processing on tryptophan, melatonin, phenolic and flavonoid contents in mulberry (Morus alba L.) leaves: quantitative analysis by LC-MS/MS,” Molecules, vol. 27, no.15, pp. 4979, Aug. 2022.
C. Yongram, S. Thapphasaraphong, P. Mahakunakorn, B. Sungthong, R. Anorach, C. Phiphatwatcharaded, S. Katekaew, and P. Puthongking, “Inhibitory effect of leaf, bark and twig of Dipterocarpus alatus on the inflammation mediators, nitric oxide, PGE2, IL-1β and TNF-α in Macrophage RAW 264.7,” Tropical Journal of Natural Product Research, vol. 5, no. 2, pp. 299-303, Feb. 2021.
P. Siriparu, P. Panyatip, T. Pota, J. Ratha, C. Yongram, T. Srisongkram, B. Sungthong, and P. Puthongking, “Effect of germination and illumination on melatonin and its metabolites, phenolic content, and antioxidant activity in mung bean sprouts,” Plants, vol. 11, no. 21, pp. 2990, Nov. 2022.
X. Chen, H. Deng, J. A. Heise, D. P. Puthoff, N. B. Abboud, H. Yu, and J. Peng, “Contents of cannabinoids in hemp varieties grown in Maryland,” ACS Omega, vol. 6, no. 47, pp. 32186-32197, Nov. 2021.
C. Yongram, P. Panyatip, P. Siriparu, J. Ratha, B. Sungthong, S. Tadtong, and P. Puthongking, “Influence of maturity stage on tryptophan, phenolic, flavonoid, and anthocyanin content, and antioxidant activity of Morus alba L. fruit,” Rasayan journal of chemistry, vol. 15, no. 3, pp. 1693-1701, Jul-Sep. 2022.
I. F. F. Benzie and J. J. Strain, “The ferric reducing ability of plasma (FRAP) as a measure of “antioxidant power”: The FRAP assay,” Analytical Biochemistry, vol. 239, no. 1, pp. 70-76, Jul. 1996.
P. M. Dewick, Medicinal Natural Products: A Biosynthetic Approach, 3rd ed. United Kingdom: John Wiley & Sons Ltd., 2009.
S. Aryal, M. K. Baniya, K. Danekhu, P. Kunwar, R. Gurung, and N. Koirala, “Total phenolic content, flavonoid content and antioxidant potential of wild vegetables from western Nepal,” Plants, vol. 8, no. 4, pp. 96, Apr. 2019.
A. J. Hampson, M. Grimaldi, J. Axelrod, and D. Wink, “Cannabidiol and (-) delta 9-tetrahydrocannabinol are neuroprotective antioxidants,” Proceedings of the National Academy of Sciences of the United States of America (PNAS), vol. 95, no. 14, pp. 8268-8273, Jul. 1998.
Z. M. C. Yazici, B. Bilge, and S. Bolkent, “Anti-inflammatory potential of delta-9-tetrahydrocannabinol in hyperinsulinemia: an experimental study,” Molecular Biology Reports, vol. 49, no. 12, pp. 11891-11899, Dec. 2022.
A. L. Dawidowicz, M. O. Tomczyk, and R. Typek, “CBG, CBD, ∆9-THC, CBN, CBGA, CBDA and ∆9-THCA as antioxidant agents and their intervention abilities in antioxidant action,” Fitoterapia, vol. 152, pp. 104915, Jul. 2021.
S. Phongpaichit, J. Nikom, N. Rungjindamai, J. Sakayaroj, N. Hutadilok-Towatana, V. Rukachaisirikul, and K. Kirtikara, “Biological activities of extracts from endophytic fungi isolated from Garcinia plants,” FEMS Immunology and Medical Microbiology, vol. 51, no. 3, pp. 517–525, Dec. 2007.
P. Pratumyam, K. Lairungruang, P. Thimkorn, S. Obhasi, and S. Sangvichien, “Antioxidant activities and total phenolic contents of plook fai thatu remedy,” Journal of Traditional Thai Medical Research, vol.9, no.1, pp. 13-27, Jan-Jun. 2023.
K. Ouncharoen and W. Leelananthakul, “Antioxidant and anti-inflammatory activities of Prasa Khamin-Oi remedy and its herbal components,” Journal of Khon Kaen Provincial Health Office, vol. 4 no. 2, pp. 225-238, Jul-Dec. 2022.
C. Threrapanithan, N. Jaiaree, A. Itharat, S. Makchuchit, P. Thongdeeying, and S. Panthong, “Anti-inflammatory and antioxidant activities of the Thai traditional remedy called “Leard-ngam” and its plant ingredients,” Thammasat Medical Journal, vol. 15, no. 3, pp. 376-383, Jul-Sep. 2015.
S. H. Nam, J. Park, W. Jun, D. Kim, J. A. Ko, A. M. Abd El-Aty, J. Y. Choi, D. I. Kim, and K. Y. Yang, “Transglycosylation of gallic acid by using Leuconostoc glucansucrase and its characterization as a functional cosmetic agent,” AMB Express, vol. 7 no. 1, pp. 224, Dec 2017.
M. A. Awang, M. A. Z. Benjamin, A. Anuar, M. F. Ismail, S. D. Ramaiya, and S. N. A. M. Hashim, “Dataset of gallic acid quantification and their antioxidant and anti-inflammatory activities of different solvent extractions from Kacip Fatimah (Labisia pumila Benth. & Hook. f.) leaves,” Data Brief. vol. 5, no. 51, pp.109644, Dec. 2003.
