Method Development for Iron Content Analysis Using Lotus Leaves Extract and Ultraviolet-Visible Spectroscopy Technique
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Abstract
This research aims to study the method development for Iron content analysis using lotus leaves extract and Ultraviolet-Visible spectroscopy. The lotus leaves extract was prepared by solvent extraction method that used acetate buffer solution as extractant. The chemical structure of lotus leaves extracts was detected by Fourier Transform Infrared Spectrometer (FT-IR). The result showed the functional groups of lotus leaves extracts were similar to tannic acid standard. The optimum condition for Iron content analysis with the developed method included acetate buffer solution (pH 4.8) used as suitable solvent, the concentration of tannic acid in lotus leaves extracts of 5.6 milligrams per liter. The reaction time was 10 minutes. The detected wavelength was 570 nanometers. The linearity range of the developed method was 1-10 milligrams per Liter. The linear equation was y = 0.0326x + 0.289. The coefficient of determination (R2) was 0.9956. The limit of detection was 0.51 milligrams per Liter. The percentage relative standard deviation (%RSD) of intra-day and inter-day were 0.14 and 1.28, respectively. The percentage recovery was 87-104. The result of iron content in pharmaceutical tablet that obtained from the developed method was well-correlated with Flame Atomic Absorption Spectroscopy. This developed method is simple, convenient, environmentally friendly, cheap and use the instrument that available in laboratory.
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ลิขสิทธ์ ของมหาวิทยาลัยเทคโนโลยีราชมงคลพระนครReferences
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