Antioxidant and Anticancer Activities of the Extract from Tiliacora triandra Diels. in Nakhonsawan Province

Main Article Content

Duangsuree - Sanseera
Boonsom Liawruangrath
Saisunee Liawruangrath


In this research, antioxidant  and anticancer activities including quantity of the extract from Tiliacora triandra Diels. in Nakhonsawan province from Mueang District, Ta Khli District, Banphot Phisai District, Kao Liao District and Lat Yao District were studied. The  aerial parts of T. triandra were macerated with hexane, chloroform and methanol, sequentially. The antioxidant and anticancer activities of the crude extract of this plant were determined. The percentage yields of the crude extract were calculated based on a dry weight. The methanol extract of this plant from  Kao Liao District gave the highest percentage yields. The antioxidant activity of the crude extract of this plant were determined using ABTS and DPPH methods. The hexane extract of the plant from  Banphot Phisai District exhibited the highest antioxidant activity (ABTS) with an   IC50 of 2.94 mg/mL. The methanol extract of the plant from Ta Khli District exhibited the highest antioxidant activity (DPPH) with an   IC50 of 0.36 mg/mL. The anticancer activity of the plant extracts were performed using the resazurin microplate assay (REMA). Only hexane extract of this plant from  Mueang District showed anticancer activity against of two cancer cell lines: KB-Oral cavity cancer and MCF7-Breast cancer with the IC50 values of 41.14 and 38.26 µg/mL, respectively.

Article Details



[1] Balandrin MF., Kinghorn AD., Farnsworth NR. Plant-derived natural products in drug discovery and development: an overview. In Human Medicinal Agents from Plants. Edited by Kinghorn AD, Balandrin MF. North Carolina, USA : Oxford University Press USA; 1993.

[2] Fumio I., Supanee D., Naoko F., et al. Chemical and biological studies on some Thai medicinal plants. J Sci Soc 1990; 16:25-31.

[3] Pavanand K., Webster HK., Yongvanitchit K. et al. Antimalarial activity of Tiliacora triandra diels against Plasmodium falciparum in vitro. Phytotherapy Research 1989; 3:215-7.

[4] Brand-Williams W., Cuvelier ME., Berset C. Use of a free radical method to evaluate antioxidant activity. Lebensmittel-Wissenschaft u-Technol 1995; 28:25-30.

[5] Roberta R., Nicoletta P., Anna P., et al. Antioxidant activity applying an improved ABTS radical cation decolorization assay. J Free Rad Biol Med 1999; 26:1231-7.

[6] Brien JO., Wilson I., Orton T. et al. Investigation of the alamar blue (resazurin) fluorescent dye for the assessment of mammalian cell cytotoxicity. Eur J Biochem 2000; 267:5421-6.

[7] Phadungkit M., Somdee T., Kangsadalampai K. Phytochemical screening, antioxidant and antimutagenic activities of selected Thai edible plant extracts. J Med Plants Res 2012; 6:662-6.