Phytochemical screening, Antioxidant and Anti-alpha(α)-glucosidase of Nelumbo nucifera Gaerth

Authors

  • Napattaorn Buachoon Faculty of Science and Technology, Valaya Alongkorn Rajabhat University under the Royal Patronage, Thailand

Keywords:

Phytochemical Screening, Antioxidant Activity, Anti-α-glucosidase, Nelumbo nucifera Gaerth

Abstract

The present study was performed to evaluate the phytochemical screening, antioxidant activity and anti-α-glucosidase of crude ethanol extracted from several parts of three sacred lotus spices including white Nelumbo nucifera Gaertn. var. pekinese, Nelumbo nucifera Gaertn. cv. roseum plenum and Nelumbo nucifera Gaertn. cv. album plenum. Phytochemical screening of the ethanoic extracted from these plant revealed that flavonoids, coumarin, saponins, tannins and terpenoids were found. The extracts were used to determine the total phenolic content (50.56±0.94 to 94.67±2.10 mg GAE/g extract), total tannin content (17.38 to 28.53 mg TAE/g extract) and total flavonoids content (24.91±0.84 to 37.32 ±1.24 mg QE/g extract). The highest antioxidant activity using DPPH free radical scavenging method at EC50 22.98 mg/mL and 25.06 mg/mL concentration was demonstrated by Nelumbo nucifera Gaertn. var. pekinese pollens extracts and petals extracts of Nelumbo nucifera Gaertn. var. pekinese respectively. In addition, the extracts of three sacred lotus spices were evaluated for anti-α-glucosidase. From these results of 2 mg/mL concentration, it was found that the extracts of the pollen and petals from Nelumbo nucifera Gaertn. var. pekinese showed the highest anti-α-glucosidase (91.49±0.25% and 70.24±0.02%).

References

Rattana S, Sungthong B. Antioxidant activities and total phenolic contents of methanolic extract from five fragrant flowers. Proceeding of 12th Mahasarakham University Conference; 2016 Aug 4-5; Mahasarakham University, Thailand. 2016. P.359-62.

Buachoon N. Chemical Compositions and Antioxidant Activity of Crude Extract from Phyllanthus emblica L. RMUTSB Academic Journal science and technology. 2017; 10(2):8-17.

Wongklang S, Steinrut L, Itharat A. Antioxidant Activity of Nelumbo nucifera Gaerth Extract. Agricultural Sci J. 2014; 45Suppl 2: 673-6.

Liu S, Li D, Huang B, et al. Inhibition of pancreatic lipase, α-glucosidase, α-amylase, and hypolipidemic effects of the total flavonoids from Nelumbo nucifera leaves. J Ethnopharmacol. 2013;149:263–9.

Ayoola GA, Coker H, Adesegun SA, et al. Phytochemical screening and antioxidant activities of some selected medicinal plants used for malaria therapy in southwestern Nigeria. Trop J Pharmaceut Res. 2008;7(3):1019–24.

Majhenič L, Škerget M, Knez Ž. Antioxidant and antimicrobial activity of guarana seed extracts. Food Chem. 2007; 104(3):1258–68.

Arvouet GA, Vennat B, Pourrat A, et al. Identification des Principaux Constituants. Journal de Pharmacie de Belgique. 1994; 9(6):462-8.

Braca A, Sortino C, Politi M, et al. Antioxidant activity of flavonoids from Liccania licaniaeflora. J Ethno-pharmacol. 2002; 79(3):379–81.

Rai S, Wahile A, Mukherjee K, et al. Antioxidant activity of Nelumbo nucifera (sacred lotus) seeds. J Ethno- pharmacol, 2006; 104(3):322–7.

Matsui T, Yoshimoto C, Osajima K. In vitro survey of alphaglucosidase inhibitory food components. Biosci Biotechnol Biochem. 1996; 60 :2019-22.

Venkatesh B, Dorai A. Antibacterial and antioxidant potential of white and pink Nelumbo Nucifera Gaertn flowers. International Conference on Bioscience, Biochemistry and Bioinformatics IPCBEE;2011 Feb 26; Singapore. 2015. P.213-7.

Buachoon N. Yawanart N. Development of Skin Lotion from Albizia Myriophylia Benth. and Ardisia Elliptica Thum. Crude Extracts. VRU Research and Development Journal Science and Technology. 2018; 12(2):74-85.

Lee HK, Choi YM, Noh DO, et al. Antioxidant effect of Korean traditional lotus liquor (Yunyupju). International Journal of Food Science and Technology. 2005; 40(7):709–715.

Kim ES, Weon JB, Yun BR, et al. Cognitive enhancing and neuro- protective effect of the embryo of the Nelumbo nucifera seed. Evidence - Based Complementary and Alternative Medicine. Evid Based Complement Alternat Med .2014; Article ID 869831, 9.

Nishibori N, Sawaguchi M, Hiroi T, et al. Inhibitory effects of aqueous extract prepared from joint part of lotus root on α-amylase and α-glucosidase activities. Phytopharmacology. 2012:3(1);1-11.

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Published

2020-06-19

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Research Articles