Total phenolic contents and antioxidant activities of sub-fractions from stem barks of Garcinia pedunculata
Total phenolic contents and antioxidant activities of sub-fractions from stem barks of Garcinia pedunculata
Keywords:
Antioxidant, Sub-fraction, Column chromatography, Garcinia pedunculata, Total phenolic contentsAbstract
The objective of this research was to compare the antioxidant activity of crude extracts and sub-fractions from the stem bark of Garcinia pedunculata. Six sub-fractions (Fr1-Fr6) were separated from ethyl acetate crude extract of Garcinia pedunculata stem bark using the column chromatography technique by the gradually increasing polarity of the eluent. The antioxidant activities of the ethyl acetate crude extract and six sub-fractions were evaluated using DPPH and ABTS techniques. It was found that, the ethyl acetate crude extract and sub-fractions 1 -3 (Fr1-Fr3) showed IC50 values greater than 100 µg/mL, while sub-fractions 4-6 (Fr4-Fr6) displayed IC50 values in the range of 43.19. -92.49 µg/mL. In addition, the antioxidant activity examined using the ABTS technique exhibited that the ethyl acetate crude extract and sub-fractions 1 -2 (Fr1-Fr2) showed IC50 values greater than 100 µg/mL while sub-fractions 3-6 (Fr3-Fr6) showed IC50 values in the range of 7.71-65.71 ug/mL. Total phenolic compounds of ethyl acetate crude extract and sub-fractions 1-6 (Fr1-Fr6) were ranged from 1.96 to 275.96 mg mgGAE/gDW. The sub-fraction 4 (Fr4) showed the highest antioxidant activities and total phenolic content.
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