Optimization and characterization of synthesis of silver nanoparticles from Manihot esculenta Crantz L. petiole and leaf aqueous extracts and its antibacterial activity
คำสำคัญ:
Manihot esculenta Crantz L., Silver nanoparticles, Green synthesis, Hot water extractionบทคัดย่อ
The objective of this study was to investigate the structure and antibacterial activity of silver nanoparticles (AgNPs) synthesized from the petioles and leaves of Manihot esculenta Crantz L. using hot water extraction (PMH and LMH extract, respectively). Optimal synthesis of AgNPs was criticized from UV-vis absorption spectra. For AgNPs synthesized from PMH extract (AgPMH), the synthesis was carried out by addition of 2 mg/mL of the PMH extract pH 12.0 with 4 mM silver nitrate solution in a 2:8 (v/v) ratio at a temperature of 60 ๐C for 120 min. For AgNPs synthesized from LMH extract (AgLMH), the synthesis was carried by the addition 2 mg/mL of LMH extract pH 10.0 with 4 mM silver nitrate solution in 2:8 (v/v) ratio at temperature 80 ๐C for 120 min. The change of Fourier-transform infrared (FTIR) spectra indicated that the AgNPs of both extracts were synthesized. Transmission electron microscopy (TEM) analysis revealed that the synthesized nanoparticles were within 15.27 to 95.58 nm in size for AgPMH and 3.98 to 23.23 nm for AgLMH. X-ray diffraction (XRD) results showed that nanoparticle formed were crystalline with face centered cubic geometry. Both synthesized AgNPs did not show activity against Escherichia coli and Bacillus cereus.
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