Antioxidant properties and the inhibition of cholangiocarcinoma cell proliferation from leaf extracts of Michelia alba and Michelia champaca
Keywords:
Cholangiocarcinoma RMCCA-1,, antioxidant, Butylated hydroxyltoluene (BHT), Michelia alba, Michelia champacaAbstract
Our research group studied the properties of crude extracts from Michelia alba and Michelia champaca leaves using the extraction method in the organic solvent i.e. hexane, ethyl acetate and methanol. The subjects of analysis were the collection of M. alba and M. champaca leaves from different time in January, April and June in 2016 which compared the antioxidant properties via DPPH scavenging assay. The results shown that the extraction of M. alba and M. champaca in methanol from all three periods composed of antioxidant properties more than the extraction M. alba and M. champaca in hexane and ethyl acetate. Additionally, the comparing of antioxidant properties with BHT, standard antioxidant agent, were found that the extraction of M. champaca in methanol from January and June can determine the IC50 to be 0.018+0.001 mg/mL. These results suggested the better antioxidant values than the standard antioxidant BHT which can determine the IC50 to be 0.048+0.002 mg/mL. Moreover, the extraction of M. champaca in ethyl acetate in January inhibited cell proliferation of Cholangiocarcinoma RMCCA-1 with IC50 of 13.51 + 1.12 μg/mL using the MTT assay method for detection. The range in the concentration of M. alba and M. champaca leaves extracts were applied to determine the effect against the cell proliferation in Cholangiocyte MMNK-1. The results shown that there is not significant affect the cell proliferation in MMNK-1. Together, results of this study show that crude extract of M. alba and M. champaca have antioxidant properties and anti-cancer cell proliferation. This study may be useful for the development of some alternative anti-cancer drug in the future. However, mechanism of inhibition of cholangiocarcinoma cell proliferation may be further elucidate.
References
คณิตา เลขะกุล. ไม้ดอกและไม้ประดับ เฉลิมพระเกียรติสมเด็จพระนางเจ้าสิริกิติ พระบรมราชินีนาถ. พิมพ์ครั้งที่ 1 กรุงเทพฯ: บริษัท ด่านสุทธาการพิมพ์ จำกัด; 2536
Silalahi M, Nisyawati W, Walujo EB, Supriatna J, Mangun W. The local knowledge of medicinal plants trader and diversity of medicinal plants in the Kabanjahe traditional market, North Sumatra, Indonesia. J Ethnopharmacol 2015;175:432-43.
Chiang HM, Chen HC, Lin TJ, Shih IC, Wen KC. Michelia alba extract attenuates UVB-induced expression of matrix metalloproteinases via MAP kinase pathway in human dermal fibroblasts. Food Chem Toxicol 2012;50(12):4260-
Mullaicharam B, Kumar MS. Effect of Michelia champaca Linn on pylorous ligated rats. J Appl Pharm Sci 2011;1(2):60-4.
Ahmad H, Sehgal S, Mishra A, Gupta R, Saraf SA. TLC detection of β-sitosterol in Michelia champaca L. leaves and stem bark and it’s determination by HPTLC. Phcog J 2012;(4):45-55.
Parimi U, Kolli D. Antibacterial and free radical scavenging activity of Michelia champaca Linn. flower extracts. Free Rad Antiox 2012;2(2):58-61.
Silva MB, Tencomnao T. The protective effect of some Thai plants and their bioactive compounds in UV light-induced skin carcinogenesis. J Photochem Photobiol 2018;185:80-9.
Yeh YT, Huang JC, Kuo PL, Chen CY. Bioactive constituents from Michelia champaca. Nat Prod Commun. 2011;6(9):1251-2.
Atjanasuppat K, Wongkham W, Meepowpan P, Kittakoop P, Sobhon P, Bartlett A, et al. In vitro screening for anthelmintic and antitumour activity of ethnomedicinal plants from Thailand. J Ethnopharmacol 2009;123(3):475-82.
Noysang C, Mahringer A, Zeino M, Saeed M, Luanratana O, Fricker G, et al. Cytotoxicity and inhibition of P-glycoprotein by selected medicinal plants from Thailand. J Ethnopharmacol. 2014;155(1):633-41.
Sirica AE. Cholangiocarcinoma: molecular targeting strategies for chemoprevention and therapy. Hepatology 2005; 41:5-15.
de Groen PC, Gores GJ, LaRusso NF, Gunderson LL, Nagorney DM. Biliary tract cancers. N Engl J Med 1999;341:1368-78.
Vatanasapt V, Sriamporn S, Vatanasapt P. Cancer control in Thailand. Jpn J Clin Oncol 2002;32 Suppl:S82-S91.
Rattanasinganchan P, Leelawat K, Treepongkaruna SA, Tocharoentanaphol C, Subwongcharoen S, Suthiphongchai T, et al. Establishment and characterization of a cholangiocarcinoma cell line (RMCCA-1) from a Thai patient. World J Gastroenterol 2006;12(40):6500-6.
Molyneux P. The use of the stable free radical diphenylpicrylhydrazyl (DPPH) for estimating antioxidant activity. Songklanakarin J Sci Technol 2004;26(2):211-19.
Ferrari M, Fornasiero MC, Isetta AM. MTT colorimetric assay for testing macrophage cytotoxic activity in vitro. J Immunol Methods 1990;131(2):165-72.
Dinesh K, Kumar S, Taprial S, Kashyap D, Kumar A, Prakash O. A review of chemical and biological profile of genus Michelia. Chin J Integr Med 2012;10(12):1336-41.
Hossain MDM, Jahangir R, Hasan SMR, Akter R, Ahmed T, Islam MDI, et al. Antioxidant, analgesic and Cytotoxic activity of Michelia champaca Linn. leaf. S J Pharm Sci. 2009;2(2):1-7.
Rajshree S, Varma R. Michelia champaca L. (Swarna Champa): a review. IJERSTE 2016;5(8):78-82.
Chen CY, Huang LY, Chen LJ, Lo WL, Kuo SY, Wang YD, et al. Chemical constituents from the leaves of Michelia alba. Chem Nat Compd. 2008;44(1):137-39.
Wei LS, Wee W, Siong JYF, Syamsumir DF. Characterization of antimicrobial, antioxidant, anticancer property and chemical composition of Michelia champaca seed and flower extracts. S J Pharm Sci. 2011;4(1):19-24.
Downloads
Published
How to Cite
Issue
Section
License
บทความทุกบทความที่ได้รับการตีพิมพ์ถือเป็นลิขสิทธิ์ของ คณะวิทยาศาสตร์แฟละเทคโนโลยี มหาวิทยาลัยหัวเฉียวเฉลิมพระเกียรติ
