The Comparison of rosmarinic acid in Orthosiphon aristatus extract by high performance liquid chromatography

Authors

  • Chonlachid kamphan Department of Thai Traditional Medicine, Faculty of Natural Resources Rajamangala University of Technology Isan, Sirindhorn College of Public health
  • Ronnachai Poowanna Department of Thai Traditional Medicine, Faculty of Natural Resources, Rajamangala University of Technology Isan
  • Panicha Pongnaratorn Department of Thai Traditional Medicine, Faculty of Natural Resources, Rajamangala University of Technology Isan
  • Pensiri Chan-ae Department of Thai Traditional Medicine, Faculty of Natural Resources, Rajamangala University of Technology Isan
  • Warin Ohnon Department of Thai Traditional Medicine, Faculty of Natural Resources, Rajamangala University of Technology Isan
  • Naruwat Pakdee Department of Thai Traditional Medicine, Faculty of Natural Resources, Rajamangala University of Technology Isan

Keywords:

Orthosiphon aristatus, Rosmarinic acid, Thin layer chromatography, High performance liquid chromatography

Abstract

The objective of this study is to compare the amount of rosmarinic acid in Orthosiphon aristatus extract from 10 different origins. The methods were used thin layer chromatography and high-performance liquid chromatography. The leaves of Orthosiphon aristatus were extracted with 70% ethanol. The results showed that crude extracts from Prachinburi province were found the highest yield at 13.12 % while Chiang Rai province had the lowest yield (6.66 %). TLC fingerprints showed a similar pattern and a spot at retardation factor (Rf) value of 0.35 was identified as standard rosmarinic acid. According to the high-performance liquid chromatography, gif.latex?\lambdamax was 329 nm and the retention time was 12.50 minutes. Nakhon Nayok province has the greatest amount of 350.86 microgram/ml (1.75%) of rosmarinic acid among the planting sites while Yala province has the lowest at 81.16 microgram/ml (0.41 %). The method validation of the HPLC technique demonstrated good linearity and represented a high correlation coefficient value (R2) were 0.9997, %recovery was 99.74 – 108.02. Intra-day and inter-day precisions were showed %RSD value 0.1 – 1.9 and 0.3 – 0.6, respectively. In addition, LOD and LOQ were 0.06 and 0.17 micrograms/milliliter, respectively. It has been demonstrated that this method can accurately and consistently analyze the rosmarinic acid in Orthosiphon aristatus.

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Published

2022-12-07

How to Cite

kamphan, C., Poowanna, R. ., Pongnaratorn, P. ., Chan-ae, P. ., Ohnon, W. ., & Pakdee, N. (2022). The Comparison of rosmarinic acid in Orthosiphon aristatus extract by high performance liquid chromatography. Huachiew Chalermprakiet Science and Technology Journal, 8(2), 81–91. retrieved from https://ph02.tci-thaijo.org/index.php/scihcu/article/view/247254

Issue

Vol. 8 No. 2 (2022): July-December 2022

Section

Research Articles

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