In Vitro Antioxidant and Cytotoxic Activities of the Mitragyna speciose Kort. leaf extract.
Keywords:
Antioxidant, Cytotoxicity, In vitro, Mitragyna speciosa Korth. (Kratom)Abstract
This research aimed to study in vitro antioxidant and cytotoxic activities of Mitragyna speciose Kort. leaf extract (Kratom leaves extract). The antioxidant properties were determined using DPPH assay and Metal chelating assay. The Kratom leaves extract was also tested for in vitro cytotoxic potential against various human cancer cell lines including HT29, KB, MCF7 and HepG2 by MTT assay. The results showed that Kratom leaves extract at various concentrations of antioxidant activity were statistically significant differences (p<0.05). It could scavenge DPPH radical (SC50= 43 µg/ml) and exert a metal chelating activity (MC50 >500 µg/ml). The cytotoxicity to human cancer cell lines showed the highest cytotoxic activity against HepG2 (IC50= 593 µg/ml) followed by KB (IC50= 1,178 µg/ml), MCF7 (IC50= 1,412 µg/ml) and HT29 (IC50= 2,343 µg/ml) respectively. Based on this research, Kratom leaf extract has the potential to be a natural antioxidant and can be developed into a therapeutic agent for treating cancer. In addition, it provides scientific evidence to support the use of Kratom in Thai wisdom for cancer treatments.
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