Bioactive activities of peptides extracted from Khao Dowk Mali 105 defatted rice bran
Keywords:
Rice bran, Peptide hydrolysate, Antioxidant activity, Anti-inflammatory activityAbstract
The aim of this research was to study the antioxidant and anti-inflammatory activities of peptide hydrolyzate from defatted Khao Dawk Mali 105 rice bran (DFRB), which is agricultural waste from the rice bran oil industry. Peptide hydrolysate from DFRB was obtained by enzymatic hydrolysis by Alcalase (0.875%). Amino acid profile, ability of antioxidant and anti-inflammatory activity of peptide hydrolysate were evaluated. The DFRB peptide hydrolysate (DFRBH) consisted of 27.66% of amino acids in the form of short polypeptides. It had ability to scavenge DPPH radical (IC50 = 1.15 mg/mL) and reduce ferric ion (FRAP) (157.12 µmol Fe2+/g). The anti-inflammatory by inhibition the production of pro-inflammatory mediators, tumor necrosis factor- (TNF-
), interleukin-6 (IL-6), interleukin-1
(IL-1
) and nitric oxide (NO) of of DFRBH aqueous solution at 5% concentration were 49.07%, 34.66%, 44.98% and 41.13% of lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophage cells. These results suggested that DFRBH could potentially be used as ingredient for functional food and therapeutic agent against oxidant and inflammatory diseases.
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