The effect of pH on levan synthesis from Bacillus siamensis using batch bio-reactor
Keywords:
Bioreactor, Levan, Polysaccharide , Bacillus siamensisAbstract
Levan is a polysaccharide synthesized from sucrose by levansucrase. The structure consists of a fructose polymer as the main chain and a single glucose molecule at the terminal end, which is linked by β-(2, 6) glycosidic bonds. It appears as a white or yellow powder and is soluble in both water and oil. It is used in biotechnology and chemical industries. However, in previous study, levan was synthesized by levansucrase from Bacillus siamensis in a laboratory scale using 250 mL batch-flask. Throughout the synthesis process, the pH was not monitored or controlled. To address the challenges of precision fermentation and scale-up, this research focuses on comparing the synthesis of levan under pH-controlled and uncontrolled conditions, utilizing batch-flask and bioreactor to investigate the effects of maintaining a controlled pH of 5.0 versus uncontrolled pH conditions on levan synthesis by B. siamensis. The results showed that the levan biosynthesis using both batch-flask and bioreactor at controlled pH of 5.0 demonstrated the growth of B. siamensis greater than uncontrolled pH (p < 0.05). On the contrary, the amount of levan produced using both batch-flask and bioreactor with uncontrolled pH were greater than controlled pH of 5.0, reaching 16.23 and 21.83 % (w/v) in batch-flask and bioreactor, respectively (p < 0.05).
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